Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells
نویسندگان
چکیده
منابع مشابه
Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures.
Treatment of suspension-cultured parsley (Petroselinum crispum) cells with fungal elicitor triggers rapid, transient and sequential phosphorylation of a number of proteins, as shown by electrophoretic analysis on two-dimensional gels. This response is rapidly reversed by removal of the elicitor from the medium and appears to be specific. It is not observed in cells exposed to other environmenta...
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The presence of three genes encoding pathogenesis-related protein 1 (PR1) in cultured parsley cells and the activation of all three genes by fungal elicitor are demonstrated. In vivo dimethyl sulfate footprinting was used to identify two putative sites of protein-DNA interaction in the promoter of one PR1 gene, located around positions -240 and -130 relative to the transcription start site. The...
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Activation of plant defense genes was investigated by analysis of transcripts completed in vitro by isolated nuclei. Elicitor treatment of suspension-cultured bean (Phaseolus vulgaris L.) cells caused marked transient stimulation of transcription of genes encoding apoproteins of cell wall hydroxyproline-rich glycoproteins (HRGP) and the phenylpropanoid biosynthetic enzymes phenylalanine ammonia...
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Treatment of cultured parsley (Petroselinum crispum L.) cells with a structurally defined peptide elicitor (Pep25) of fungal origin has previously been shown to cause rapid and large changes in the levels of various desaturated fatty acids. We isolated two distinct parsley cDNAs sharing high sequence similarity with microsomal w-6 fatty acid desaturases (FADs). One of them was functionally iden...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.8.2427